Anti-NGAL (Monkey, neutrophil gelatinase-associated lipocalin)
Mouse monoclonal antibody
Cat.No. ABS 038-23
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4,containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). The expression and release of NGAL from renal tubules are dramatically increased by ischemic or nephrotoxic injury (3).
| Recombinant human NGAL adsorbed onto aluminum hydroxide gel |
|
ABS 038-23 binds cynomolgus monkey (Macaca fascicularis) NGAL and cross-reacts with human NGAL. |
Not determined
ABS 038-23 (as capture antibody) forms a sandwich pair with HYB 211-02 (as biotinylated detection antibody) for measuring cynomolgus monkey (Macaca fascicularis) and human NGAL. ABS 038-23 binds human NGAL in solution.
The ELISA dilution guideline of 1/30,000 applies to recombinant human NGAL coated directly at 0.8 µg/mL.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/30.000 | |
| Immunoblotting | ND | ||
| Immunohistochemistry | ND |
1. Kjeldsen L, Johnsen AH, Sengeløv H, Borregaard N (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem 268:10425-10432.
2. Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M, Kjeldsen L (1996) Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel diseases. Gut 38:414-420.
3. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P (2003) Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14:2534-2543.
Downloads
ABS 038-23.pdf (20.06 kB) ABS 038-23 product specificification sheet
Monkey NGAL.pdf (650.73 kB)
Monkey NGAL flyer
